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2-Mercaptoethanol, thioglycolic acid, glutathione, 3-mercapto-1,2-propanediol and 3-mercapto-2-butanol showed catalytic activities on the hydrolysis of alpha-aminophenylacetonitrile to phenylglycineamide at the rate of 12.19 X 10-2, 8.03 X 10-2 , 6.83 X 10-2, 8.60 X 10-2 and 6.04 X 10-2mM min-1, respectively. The hydrolysis rate was faster in buffer than in water. The hydrolysis of the nitrile compound to phenylglycine was limited.
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